10091 Paqr4 Status: ES cell colonies screened / QC one positive
| Gene | Paqr4 |
| Chromosome | 17 |
| Deletion Start | 23,740,022 |
| Deletion End | 23,737,262 |
| Deletion Size | 2,761 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,739,703 | 23,739,722 | GTCCCAAGCTTCGTTGCCAG |
| TUP | 23,739,677 | 23,739,701 | TCCTGCTTTCCCAGTAGTGCCCCAA |
| TUR | 23,739,656 | 23,739,675 | ACTCTGCTCCCCTGTCCTGC |
| TDF | 23,737,642 | 23,737,661 | ATGCACTTGCTGAGTGTGGG |
| TDP | 23,737,618 | 23,737,640 | TCCATCCTCCAGCTCCATGCTGG |
| TDR | 23,737,597 | 23,737,616 | CAGAGCAGGTCAGGCACAAC |
PCR Primers
| Primer | Sequence |
| SU | TGGAGCTGAATGCACTGAGC |
| SD | CTAGGAAAAGCAGCAGTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 397 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
