10096 Oscar Status: Cancelled
| Gene | Oscar |
| Chromosome | 7 |
| Deletion Start | 3,616,074 |
| Deletion End | 3,610,764 |
| Deletion Size | 5,331 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,615,596 | 3,615,614 | CGGTGGCAGGCTTCATGTA |
| TUP | 3,615,577 | 3,615,594 | TCAGAGTCTAGGGCTCCT |
| TUR | 3,615,555 | 3,615,575 | GTGGACTACCGTCTCCCCTCT |
| TDF | 3,610,904 | 3,610,922 | TTTTGGCTCGCAGGTTCTG |
| TDP | 3,610,886 | 3,610,902 | CTCCTTGGACTATACCC |
| TDR | 3,610,860 | 3,610,877 | CAGCCCCAAACGGATGAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGTGCTGAGGCTACTCTG |
| SD | AGAAATTTTTGCTGGGTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 402 bp | - | + | + |
| TUF | TUR | 60 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | 405 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
