10100 Btnl9 Status: ES cell colonies screened / QC no positives
| Gene | Btnl9 |
| Chromosome | 11 |
| Deletion Start | 49,183,068 |
| Deletion End | 49,169,035 |
| Deletion Size | 14,034 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,182,875 | 49,182,894 | TTCTGCTGGGTGCCCTCTAG |
| TUP | 49,182,845 | 49,182,871 | TGGGCTCTCATGATACAGCACCAGACC |
| TUR | 49,182,800 | 49,182,820 | CCAAAACCCTGAGTGGGAATC |
| TDF | 49,169,188 | 49,169,206 | GCTCACGACGGCAGTGAAC |
| TDP | 49,169,158 | 49,169,185 | CCGGATCCTATGACCATCTGCTCTTTGC |
| TDR | 49,169,137 | 49,169,156 | GGACCTGTGGCCCTCTAACC |
PCR Primers
| Primer | Sequence |
| SU | CCAAACAATACGTTGCTTCC |
| SD | ACTATACAGAACTATACACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 324 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
