10102 Tmem74 Status: ES cell colonies screened / QC no positives
| Gene | Tmem74 |
| Chromosome | 15 |
| Deletion Start | 43,867,642 |
| Deletion End | 43,866,709 |
| Deletion Size | 934 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,867,243 | 43,867,264 | CCATCAGCCAAAGGCAGTAATC |
| TUP | 43,867,223 | 43,867,240 | CCTGTAGATCTGGGATGG |
| TUR | 43,867,189 | 43,867,210 | TGGCAGTTTCATGAGACCATTC |
| TDF | 43,867,021 | 43,867,043 | GGTGACAGTGGATCCCAATACAG |
| TDP | 43,866,996 | 43,867,016 | AGGCGTTCCATTTCCCGGGCT |
| TDR | 43,866,972 | 43,866,994 | CCTAGCATTGCACTCTCCTTTTC |
PCR Primers
| Primer | Sequence |
| SU | CGAGTTTCTCACTGCTAACC |
| SD | AGAAGTAGAAACACATAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 325 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
