10104 Adamts12 Status: Cancelled
| Gene | Adamts12 |
| Chromosome | 15 |
| Deletion Start | 11,065,014 |
| Deletion End | 11,071,812 |
| Deletion Size | 6,799 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 11,065,234 | 11,065,254 | AGGCAGGGCTGAACTCTAAGC |
| TUP | 11,065,275 | 11,065,299 | ATCCGAGCCTGAGCACACAGCAGTT |
| TUR | 11,071,613 | 11,071,633 | CAGAACCACCTGCAGCTCTCT |
| TDF | 11,071,555 | 11,071,573 | GCTGGTGGCCATGTTCTGT |
| TDP | 11,071,578 | 11,071,602 | TGGCCTGCATCACCCTGTCACTAGC |
| TDR | 11,071,613 | 11,071,633 | CAGAACCACCTGCAGCTCTCT |
PCR Primers
| Primer | Sequence |
| SU | GCTGTCAGCTCATTCACTCC |
| SD | AGAGTGTATCAACCAAGCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 343 bp | - | + | + |
| TUF | TUR | 6400 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 448 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
