10105 Adamts19 Status: Cancelled
| Gene | Adamts19 |
| Chromosome | 18 |
| Deletion Start | 58,836,790 |
| Deletion End | 58,838,003 |
| Deletion Size | 1,214 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,836,952 | 58,836,972 | GCACACAGGAAGGCAGAACTG |
| TUP | 58,836,977 | 58,837,000 | AGCTCTGCTCAACACGCACGGGGT |
| TUR | 58,837,006 | 58,837,026 | GCAAGCTGCTTCACCGCTATC |
| TDF | 58,837,289 | 58,837,308 | TCGTGGCTTGCAGGAGTTTC |
| TDP | 58,837,312 | 58,837,338 | CACGAAAGCGAGACCACCCGTCTAGTT |
| TDR | 58,837,353 | 58,837,374 | CTCAGGCACAAGGTCCATACAG |
PCR Primers
| Primer | Sequence |
| SU | GCCTGGAAACTCCTGCACTG |
| SD | AGTAAGACTTCTTCTGTCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 381 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
