10106 Fam5b Status: Cancelled
| Gene | Fam5b |
| Chromosome | 1 |
| Deletion Start | 158,268,006 |
| Deletion End | 158,246,179 |
| Deletion Size | 21,828 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 158,267,356 | 158,267,378 | TGAGTTACTGACCCCTCCTTCCT |
| TUP | 158,267,327 | 158,267,354 | ACTGTGACGATTGGTAACCAGCCAAACC |
| TUR | 158,267,295 | 158,267,316 | AGAGGGAGATTCACCCAAGTTG |
| TDF | 158,246,325 | 158,246,344 | GTCCGGCTCGACCTTTTCTC |
| TDP | 158,246,301 | 158,246,323 | TGCTTGCTCCGGCATAGGCTCAA |
| TDR | 158,246,276 | 158,246,294 | CCTGCCCACCTCATTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGCCTTGGGAAGATGCTGAGC |
| SD | CTTAGCTCTTCTTCAACTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 524 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 379 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
