| Gene | St8sia6 |
| Chromosome | 2 |
| Deletion Start | 13,793,441 |
| Deletion End | 13,792,791 |
| Deletion Size | 651 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 13,793,337 | 13,793,355 | TGCCCGCTCCAGGTAATTC |
| TUP | 13,793,307 | 13,793,331 | CCTCAACTCGCCCCACCTGAAAGGT |
| TUR | 13,793,263 | 13,793,281 | CGTCCCCTGGAAGCAACAC |
| TDF | 13,793,308 | 13,793,326 | ACTCGCCCCACCTGAAAGG |
| TDP | 13,793,267 | 13,793,291 | CCCTGGAAGCAACACCCAGAGCACA |
| TDR | 13,793,240 | 13,793,259 | TCAGTCACCCGGATCTTTCC |
PCR Primers
| Primer | Sequence |
| SU | CAGCTTCGCCAGCTTCGGC |
| SD | CGATCTCAACACAGATGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 395 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 87 bp | + | + | - |
| NeoFwd | SD | 509 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10107C-B10 | VGB6 | C57BL/6NTac | |
| 10107C-B3 | VGB6 | C57BL/6NTac | |
