| Gene | Fam171b |
| Chromosome | 2 |
| Deletion Start | 83,853,576 |
| Deletion End | 83,880,451 |
| Deletion Size | 26,896 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 83,853,583 | 83,853,604 | GGAAAACTGGAAGGATGCCAAG |
| TUP | 83,853,606 | 83,853,636 | TTCACAAATGCAAAACACCACTGAAGGACTT |
| TUR | 83,853,638 | 83,853,661 | CCAACAGATGGAGAAATCATCTGC |
| TDF | 83,879,821 | 83,879,842 | CTCAGATTGGAGCCGATATTCC |
| TDP | 83,879,846 | 83,879,874 | CCAGGAACAGACACAGACTCCAACAAGCT |
| TDR | 83,879,883 | 83,879,903 | GGGTCATCACCACAGCTTCAT |
PCR Primers
| Primer | Sequence |
| SU | TAAGTTCGACCTGATTCAGTTTC |
| SD | GAGGAACAGGCCAGAGAAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 483 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 260 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10109A-A5 | VGB6 | C57BL/6NTac | |
| 10109A-E11 | VGB6 | C57BL/6NTac | |
