| Gene | Adam29 |
| Chromosome | 8 |
| Deletion Start | 55,873,414 |
| Deletion End | 55,871,107 |
| Deletion Size | 2,308 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,873,342 | 55,873,365 | TGTTTGGGATTTTCCTGTGTTTTC |
| TUP | 55,873,313 | 55,873,340 | TGGACTCACAAAGGCTGGACATCTGCAC |
| TUR | 55,871,931 | 55,871,950 | GAGACCCCACAGGGAATTCC |
| TDF | 55,871,992 | 55,872,012 | ACCTTCCAGAGTGGTGCAATG |
| TDP | 55,871,960 | 55,871,988 | CGTCCCATGAATGTCCAGATGATGCTTAT |
| TDR | 55,871,931 | 55,871,950 | GAGACCCCACAGGGAATTCC |
PCR Primers
| Primer | Sequence |
| SU | TTCTCCCAACCCTGCTTTCA |
| SD | ATATACATATGTCTATTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 304 bp | - | + | + |
| TUF | TUR | 1435 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 306 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10114C-B8 | VGB6 | C57BL/6NTac | |
| 10114C-C8 | VGB6 | C57BL/6NTac | |
