Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

10117 Adig Status: ES cell colonies screened / QC positives

Gene	Adig
Chromosome	2
Deletion Start	158,502,726
Deletion End	158,505,907
Deletion Size	3,182
Genome Build	38

MGI ID	MGI:2675492
NCBI Gene ID	246747
BAC ID	70K16
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 2

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	158,502,817	158,502,837	GGCTTTGCTGCTGTTCTTGAC
TUP	158,502,865	158,502,890	ATCCAGCCTGAACCAGCTCACCTTGA
TUR	158,502,892	158,502,912	CCTGCCCAGCTCCAAAATTAG
TDF	158,505,683	158,505,703	GGTCTCATACAGGGCCACATG
TDP	158,505,709	158,505,733	CAGGCTTGGCACATGTCTCCTCTGG
TDR	158,505,744	158,505,764	GGGTGGGAACAGCCTGTAGTG

PCR Primers

Primer	Sequence
SU	TGACTCCTGGGTCAGGCCAGC
SD	ATGTTCCAAAAACCCAGATC
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	375 bp	-	+	+
TUF	TUR	96 bp	+	+	-
TDF	TDR	82 bp	+	+	-
NeoFwd	SD	347 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
10117A-B1	VGB6	C57BL/6NTac
10117A-B5	VGB6	C57BL/6NTac
10117A-E3	VGB6	C57BL/6NTac