| Gene | Il19 |
| Chromosome | 1 |
| Deletion Start | 130,939,238 |
| Deletion End | 130,933,036 |
| Deletion Size | 6,203 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 130,938,510 | 130,938,529 | TCAGTGGAATCAGGCTGGTG |
| TUP | 130,938,481 | 130,938,505 | AACACGCTTGCTCACTGTCCCTGGA |
| TUR | 130,938,460 | 130,938,479 | TCTGCCTGTCCTTTGCATCC |
| TDF | 130,933,797 | 130,933,816 | GTGGCCCTATCCCAGAATCC |
| TDP | 130,933,763 | 130,933,793 | CCACCTACAAGATGTCCCACCTCCTATTTCC |
| TDR | 130,933,742 | 130,933,761 | TGAGGGCCTATCGCTTAGGC |
PCR Primers
| Primer | Sequence |
| SU | GACACATGTGTAGGTGTCAC |
| SD | TGAGATCTCTCAACAGCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10119C-C2 | VGB6 | C57BL/6NTac | |
| 10119D-G12 | VGB6 | C57BL/6NTac | |
| 10119F-A1 | VGB6 | C57BL/6NTac | |
| 10119H-H8 | VGB6 | C57BL/6NTac | |
