| Gene | Islr2 |
| Chromosome | 9 |
| Deletion Start | 58,199,972 |
| Deletion End | 58,197,665 |
| Deletion Size | 2,308 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,199,872 | 58,199,891 | AAGTACGCCCACCAGTTTGC |
| TUP | 58,199,844 | 58,199,867 | CTCGCGCAGCTCCTTGTAGGCACA |
| TUR | 58,199,820 | 58,199,839 | GTTGGCTGGCAGTCCTTCTG |
| TDF | 58,198,382 | 58,198,402 | GGCCACTGCGCCTACTCTATC |
| TDP | 58,198,337 | 58,198,361 | CCACTCGTGACCACTGAACTGCCGT |
| TDR | 58,198,308 | 58,198,328 | GCGAAACCAGTAGGCATTGAC |
PCR Primers
| Primer | Sequence |
| SU | AGAAAGAAGGGTCTGTATGG |
| SD | TTTCGTGGATCGCCGTGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 422 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 340 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10121A-A9 | VGB6 | C57BL/6NTac | |
| 10121A-B2 | VGB6 | C57BL/6NTac | |
| 10121A-D7 | VGB6 | C57BL/6NTac | |
| 10121A-D12 | VGB6 | C57BL/6NTac | |
| 10121A-F8 | VGB6 | C57BL/6NTac | |
| 10121A-G11 | VGB6 | C57BL/6NTac | |
