10122 Tnfsf15 Status: ES cell colonies screened / QC no positives
| Gene | Tnfsf15 |
| Chromosome | 4 |
| Deletion Start | 63,745,027 |
| Deletion End | 63,729,570 |
| Deletion Size | 15,458 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,744,997 | 63,745,015 | GGGTTGGGCTTCGGAGAAG |
| TUP | 63,744,972 | 63,744,995 | TCCGGCAGCACTTCCACTGGGACT |
| TUR | 63,744,946 | 63,744,966 | TGGCCTCTGGCCTGTGTCTAC |
| TDF | 63,729,962 | 63,729,982 | TGACCTAGGGATGGCCTTCAC |
| TDP | 63,729,943 | 63,729,960 | AAGAACGGGATGAAGTAC |
| TDR | 63,729,920 | 63,729,940 | GGGATCACCAGGGATTTGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGTTGTAGGCGGCGCATTCC |
| SD | CATTGGATGAGGCGTGTTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 299 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 63 bp | + | + | - |
| NeoFwd | SD | 333 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
