10125 Ano7 Status: Cancelled
| Gene | Ano7 |
| Chromosome | 1 |
| Deletion Start | 93,375,125 |
| Deletion End | 93,403,024 |
| Deletion Size | 27,900 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 93,375,152 | 93,375,171 | GGCAAGCGCGAGAAGAAGAC |
| TUP | 93,375,174 | 93,375,196 | AGGCCATGTCGATCAGCACCACG |
| TUR | 93,375,200 | 93,375,219 | GCATCCATTCCCTGCTTCAG |
| TDF | 93,402,649 | 93,402,668 | GTTCTCCATTGGCCGTGTTC |
| TDP | 93,402,676 | 93,402,702 | CTTGGTTCCTGACATCCCAGAATCCGT |
| TDR | 93,402,704 | 93,402,724 | TTCCCGCTTCACCTTGATCTC |
PCR Primers
| Primer | Sequence |
| SU | AAATACCAGCTATCATTCAG |
| SD | AGTCCTAAGGAACATGTAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 370 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
