10126 Kcnj14 Status: Cancelled
| Gene | Kcnj14 |
| Chromosome | 7 |
| Deletion Start | 45,820,076 |
| Deletion End | 45,817,597 |
| Deletion Size | 2,480 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 45,819,180 | 45,819,200 | CAGCTCCCAGCAACATTAAGG |
| TUP | 45,819,154 | 45,819,178 | CAGGCTGTTGCACAGGCACTTCCCT |
| TUR | 45,819,130 | 45,819,149 | CCCCAACACCCACTCAACAC |
| TDF | 45,817,871 | 45,817,889 | TCGCCACTTCCATCGAACA |
| TDP | 45,817,844 | 45,817,869 | ATGAGGTCCCAGGGACACCAGTCTGC |
| TDR | 45,817,819 | 45,817,837 | CCCGTTCATCCAGCTCCTT |
PCR Primers
| Primer | Sequence |
| SU | TGCAGAAGACAGTGTTGTGG |
| SD | CCCAGCCTCCACGCCTTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 258 bp | - | + | + |
| TUF | TUR | 71 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 356 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
