| Gene | Aspn |
| Chromosome | 13 |
| Deletion Start | 49,551,667 |
| Deletion End | 49,566,684 |
| Deletion Size | 15,037 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 49,551,693 | 49,551,712 | GGCTGTGTGCTCTGCCAAAC |
| TUP | 49,551,714 | 49,551,741 | CTTCTTTAGCCCTTCCCACACAGCACTG |
| TUR | 49,551,750 | 49,551,774 | TGTGTCTTCCATATCCTTCAACATC |
| TDF | 49,566,556 | 49,566,577 | CTGGGAAATACAACCTGCAACA |
| TDP | 49,566,579 | 49,566,597 | TTCGTTGTGTTCTTGGCAG |
| TDR | 49,566,600 | 49,566,621 | ACATTCCCAAGCTGAACACTCA |
PCR Primers
| Primer | Sequence |
| SU | CCATGGACAGACCCAGCTG |
| SD | TCAAAATGCTGTTCTTGCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | 406 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10129A-D2 | VGB6 | C57BL/6NTac | |
| 10129A-G2 | VGB6 | C57BL/6NTac | |
