10132 Fam70a Status: Cancelled
| Gene | Fam70a |
| Chromosome | X |
| Deletion Start | 38,252,298 |
| Deletion End | 38,224,638 |
| Deletion Size | 27,661 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 38,251,894 | 38,251,916 | CATCAAAACACTTCCTCCAAACC |
| TUP | 38,251,868 | 38,251,891 | TGCCTTTGCTTTCGATTCGACGCT |
| TUR | 38,251,834 | 38,251,859 | GATCTTAGAAGTGATGTGGACTTTGC |
| TDF | 38,225,069 | 38,225,090 | GAGGGCTTTGAGGAACCTTACC |
| TDP | 38,225,050 | 38,225,067 | AGGCAGTTAAAGAGGTAC |
| TDR | 38,225,021 | 38,225,042 | GGCCATTACATGCACACTGAAG |
PCR Primers
| Primer | Sequence |
| SU | GCACTTGGGCTGCAGCTCTC |
| SD | GAATCTATGACAGACCAATAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 274 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 70 bp | + | + | - |
| NeoFwd | SD | 283 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
