| Gene | Podxl2 |
| Chromosome | 6 |
| Deletion Start | 88,865,155 |
| Deletion End | 88,848,786 |
| Deletion Size | 16,370 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,864,825 | 88,864,844 | TGTTTTGAGCCCAGGCACTC |
| TUP | 88,864,804 | 88,864,820 | ATACAGCCTGAGGCTGA |
| TUR | 88,864,781 | 88,864,799 | CAAGCTGCTCGCTGCTCTG |
| TDF | 88,849,051 | 88,849,070 | CCCATGTGCCCAAGTCATTG |
| TDP | 88,849,024 | 88,849,049 | CTTCCTCCTGCGGCCTGTGTGTATGA |
| TDR | 88,848,994 | 88,849,014 | TTGGCCCTATGCACTGTGAAC |
PCR Primers
| Primer | Sequence |
| SU | GAAGACTCAACACACTACGG |
| SD | TAAGTGGTAGGTGCTGCTTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 373 bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 271 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10133A-F2 | VGB6 | C57BL/6NTac | |
| 10133B-B12 | VGB6 | C57BL/6NTac | |
| 10133B-D5 | VGB6 | C57BL/6NTac | |
| 10133B-D8 | VGB6 | C57BL/6NTac | |
| 10133B-G5 | VGB6 | C57BL/6NTac | |
