10135 Vstm2b Status: Cancelled
| Gene | Vstm2b |
| Chromosome | 7 |
| Deletion Start | 40,900,002 |
| Deletion End | 40,929,462 |
| Deletion Size | 29,461 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,900,703 | 40,900,725 | TACCTACAGTGTCCCCAGAATCC |
| TUP | 40,900,737 | 40,900,762 | CCTGTTCTCATCCTCCTCTGGCACGT |
| TUR | 40,900,766 | 40,900,787 | GGCCTTGACAGTCTGGTATGTG |
| TDF | 40,928,689 | 40,928,711 | TTGCAACATCTGGACACTTTGAG |
| TDP | 40,928,715 | 40,928,739 | TAGGCCACTGCTCAGCTCCTCACCG |
| TDR | 40,928,743 | 40,928,766 | CCATGCTTGTCTTCAACATTTCTC |
PCR Primers
| Primer | Sequence |
| SU | GAACTTTGCTGGAGCCTCTC |
| SD | GAAGATTCAGAGGGAATGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 402 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
