10137 Tmem25 Status: Cancelled
| Gene | Tmem25 |
| Chromosome | 9 |
| Deletion Start | 44,798,730 |
| Deletion End | 44,794,956 |
| Deletion Size | 3,775 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,798,489 | 44,798,509 | GGCCTGCTGCCTCTCTGTAAC |
| TUP | 44,798,455 | 44,798,479 | CTGTGGTCTCTCCAAGTGCCTCCCA |
| TUR | 44,798,422 | 44,798,441 | CTCCCCTTGACCTGGAACAC |
| TDF | 44,795,286 | 44,795,305 | TGGGCACCACTCCATTTGTC |
| TDP | 44,795,261 | 44,795,284 | CCGCTCTGTTGCTTTCCCTGTGGG |
| TDR | 44,795,240 | 44,795,259 | GCTGCTATGCTGAGCCATTG |
PCR Primers
| Primer | Sequence |
| SU | TCATCTACTGTCAGTGCTGG |
| SD | GGCCCCAAACTCAGTGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
