10139 Tmem66 Status: ES cell colonies screened / QC no positives
| Gene | Tmem66 |
| Chromosome | 8 |
| Deletion Start | 34,154,628 |
| Deletion End | 34,170,131 |
| Deletion Size | 15,504 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,155,097 | 34,155,116 | CATCATCGCCCACTGTTGTC |
| TUP | 34,155,118 | 34,155,140 | CGAGCCATAGCAACAGGCAGCCA |
| TUR | 34,155,142 | 34,155,161 | CCACTGGGTGCATGGTAACG |
| TDF | 34,169,886 | 34,169,906 | GCCTTCCTGAGAGCAATTGTG |
| TDP | 34,169,918 | 34,169,942 | TGAGACCCAACAAGGCAGACCCTCA |
| TDR | 34,169,950 | 34,169,968 | GCAGCACGACGTGGAACAC |
PCR Primers
| Primer | Sequence |
| SU | CACTCGCTAGGGTACCACG |
| SD | GCACAATGTCAGCATTCCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 339 bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
