10140 Serpina3n Status: Cancelled
| Gene | Serpina3n |
| Chromosome | 12 |
| Deletion Start | 104,408,654 |
| Deletion End | 104,413,606 |
| Deletion Size | 4,953 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 104,409,488 | 104,409,514 | GGCATGTAGTCAACATAAACCCTAATC |
| TUP | 104,409,518 | 104,409,543 | CAGCCCAGGTCAGCCCTCTTAGATGG |
| TUR | 104,409,545 | 104,409,572 | GTGTTCTGTTGGTAACTAAGGCTAAGTC |
| TDF | 104,412,491 | 104,412,513 | CAAAGAAGCACTTGGTGGTTCAG |
| TDP | 104,412,515 | 104,412,539 | CTCACCATCCACGTTCAGGGCACAA |
| TDR | 104,412,551 | 104,412,570 | ACGTGTGCCAATCCCACATG |
PCR Primers
| Primer | Sequence |
| SU | ACTTCCTCCGTGATAGTAGG |
| SD | TTCAGGCTCAGGAACATGGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 313 bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 307 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
