10142 Paqr7 Status: ES cell colonies screened / QC no positives
| Gene | Paqr7 |
| Chromosome | 4 |
| Deletion Start | 134,506,826 |
| Deletion End | 134,507,897 |
| Deletion Size | 1,072 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 134,507,221 | 134,507,241 | TCGGAGTTCTGGCATTACAGC |
| TUP | 134,507,245 | 134,507,266 | CACCCACATAGTCCAAGAAGAA |
| TUR | 134,506,946 | 134,506,964 | GGCTTCCAGAAAAGTGGCG |
| TDF | 134,507,591 | 134,507,611 | TTTTCTCCACGGTTATGCCTG |
| TDP | 134,507,614 | 134,507,638 | AGTTGGTTCCCCGGCAGCTGTCACA |
| TDR | 134,507,640 | 134,507,658 | TTGGTGTCCCTGCCCAAAG |
PCR Primers
| Primer | Sequence |
| SU | AGCGTGACTCCTTGATCTTC |
| SD | TCAGGAGTCCTTCATCTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 279 bp | - | + | + |
| TUF | TUR | 296 bp | + | + | - |
| TDF | TDR | 68 bp | + | + | - |
| NeoFwd | SD | 294 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
