10143   Cd163     Status: ES cell colonies screened / QC positives

GeneCd163
Chromosome6
Deletion Start124,254,756
Deletion End124,279,391
Deletion Size24,636
Genome Build37
MGI IDMGI:2135946
NCBI Gene ID93671
BAC ID302b9
BAC LibraryBAC ES release 2
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 6

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF124,255,547124,255,567GCTCAGGCTTAAGAAGCTGCC
TUP124,255,576124,255,600TTGCCTCCATGCCCAGCGTTTTCAC
TUR124,255,608124,255,626TACGTGCAGCCCAGGAGTG
TDF124,278,731124,278,750TCATTCCAGGAGAAGTGCCC
TDP124,278,756124,278,782TTCATTGTCCTCACTCCTCGGGAACCT
TDR124,278,784124,278,803CCAAGGCAAATCCCTCTCAG

PCR Primers

PrimerSequence
SUGTGTTCCAAAGTGGGAGGAG
SDATGAAGATGCAGCTGTGGTC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTCATTCTCAGTATTGTTTTGCC
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev332 bp-++
TUFTUR62 bp++-
TDFTDR54 bp++-
NeoFwdSD379 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10143B-A3VGB6C57BL/6NTac
10143B-F4VGB6C57BL/6NTac
10143C-F11VGB6C57BL/6NTac
10143E-B5VGB6C57BL/6NTac
10143F-E2VGB6C57BL/6NTac
10143F-B6VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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