10144 Pdgfd Status: Cancelled
| Gene | Pdgfd |
| Chromosome | 9 |
| Deletion Start | 6,168,778 |
| Deletion End | 6,168,890 |
| Deletion Size | 113 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,168,781 | 6,168,798 | GTCGCTGAGCCCAAATGC |
| TUP | 6,168,801 | 6,168,829 | CGGCTCGTTTTAGTCTCCATTCTCCTGTG |
| TUR | 6,168,833 | 6,168,855 | TGTCCGGATAGCAGCTAAAGTTC |
| TDF | 6,168,795 | 6,168,815 | ATGCAACGGCTCGTTTTAGTC |
| TDP | 6,168,817 | 6,168,842 | CCATTCTCCTGTGCGCGAACTTTAGC |
| TDR | 6,168,845 | 6,168,865 | GTCGCAAAAGTGTCCGGATAG |
PCR Primers
| Primer | Sequence |
| SU | GAATCCACGTCAACCTGTTG |
| SD | AGGGTGAGGAGCAGGCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 273 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
