10145 Mamdc2 Status: Cancelled
| Gene | Mamdc2 |
| Chromosome | 19 |
| Deletion Start | 23,448,055 |
| Deletion End | 23,447,568 |
| Deletion Size | 488 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 23,447,894 | 23,447,916 | TGGACAATTCTCAGCCACTAAGC |
| TUP | 23,447,864 | 23,447,887 | CTCTCCCTGGTCAAGCACGGGATG |
| TUR | 23,447,838 | 23,447,857 | CCTCTGCGCAACTCCATACC |
| TDF | 23,447,715 | 23,447,734 | CCAGGCAGAAACCCAAACTG |
| TDP | 23,447,691 | 23,447,713 | AAGTCCCTTTGTGCCCGCAGCCT |
| TDR | 23,447,662 | 23,447,680 | GGGCAGGTCTAGGGCATTG |
PCR Primers
| Primer | Sequence |
| SU | AGGCACCACTTGCTGACCTC |
| SD | GCTCAGAGCCAGGTACACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 411 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 305 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
