10146 Pkd2l1 Status: ES cell colonies screened / QC no positives
| Gene | Pkd2l1 |
| Chromosome | 19 |
| Deletion Start | 44,157,714 |
| Deletion End | 44,148,488 |
| Deletion Size | 9,227 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,157,115 | 44,157,135 | CCGGGAGGACATTTTGAACTG |
| TUP | 44,157,077 | 44,157,103 | ACTCGCCGGACAAAGAAGATCAGCTCC |
| TUR | 44,157,055 | 44,157,075 | GTGCCGTTCTGAGGTCCAAAG |
| TDF | 44,149,178 | 44,149,199 | TGAAGATGCTGGAGAGGAAAGG |
| TDP | 44,149,142 | 44,149,164 | TCCCCAGGAATGGTGAGTGCCCT |
| TDR | 44,149,121 | 44,149,140 | GAACTCAGCGGACCCAACTG |
PCR Primers
| Primer | Sequence |
| SU | TTTCCCTAAAACATCTCCTG |
| SD | TCCATAAAGAAACACCAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 331 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
