10147 Ptprt Status: Cancelled
| Gene | Ptprt |
| Chromosome | 2 |
| Deletion Start | 162,278,295 |
| Deletion End | 162,253,744 |
| Deletion Size | 24,552 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 162,278,165 | 162,278,185 | CCGGGAGCCTTGAATGTCTAC |
| TUP | 162,278,139 | 162,278,163 | CCTTGGGGTCCACCATTCACCTTCA |
| TUR | 162,278,104 | 162,278,123 | CAGTGACGACGCCAGATACG |
| TDF | 162,254,018 | 162,254,038 | AATGGGCCTCATGGTGTAGTG |
| TDP | 162,253,991 | 162,254,016 | CCCATGTGGTCTCATCTGAACCTGGC |
| TDR | 162,253,957 | 162,253,977 | GCCTGGAGATCCTGGAATGTG |
PCR Primers
| Primer | Sequence |
| SU | TCTTCAGAGTCAACCTGACC |
| SD | GCCAACTTAGCTGAACAGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 272 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
