10149 Plxna2 Status: Cancelled
| Gene | Plxna2 |
| Chromosome | 1 |
| Deletion Start | 194,643,745 |
| Deletion End | 194,649,629 |
| Deletion Size | 5,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 194,643,992 | 194,644,011 | CCATCCAGGTGGCTCACAAG |
| TUP | 194,644,013 | 194,644,038 | AGCCTTGTTGTCCTCTTCTGGCCCTG |
| TUR | 194,644,053 | 194,644,073 | TCACTGCAGGGCTGTACAATG |
| TDF | 194,649,468 | 194,649,488 | GATGATAACTTCTGCGGCCTG |
| TDP | 194,649,494 | 194,649,518 | CAACCAGCCTCTGGGAGGCTCCACT |
| TDR | 194,649,520 | 194,649,539 | CAGGGTCAGTCCCTCCACAG |
PCR Primers
| Primer | Sequence |
| SU | GATTCCCTTAGCAACCTTGG |
| SD | ATCTCAATGTCTGGCAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 342 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 313 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
