10150 Ptpn18 Status: ES cell colonies screened / QC no positives
| Gene | Ptpn18 |
| Chromosome | 1 |
| Deletion Start | 34,459,809 |
| Deletion End | 34,473,648 |
| Deletion Size | 13,840 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 34,460,028 | 34,460,046 | CTCCTGCTCTCGGCTTTCC |
| TUP | 34,460,061 | 34,460,086 | TCTCACCCTGACTCTCCCAGCCTTCC |
| TUR | 34,460,088 | 34,460,106 | GGCACCTGTGGGCAAAGTG |
| TDF | 34,473,516 | 34,473,536 | GTGGGTTTGGAGGAGGTTACC |
| TDP | 34,473,541 | 34,473,568 | CACTCCTTAGCTTCTGCATGGAGCCCTT |
| TDR | 34,473,573 | 34,473,592 | GCGCAAGTTGAAGCCTAAGG |
PCR Primers
| Primer | Sequence |
| SU | AGGCGCCAGTGGGGAATTG |
| SD | CAGAATAGATCCACCACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 348 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 299 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
