10151 Gpr45 Status: ES cell colonies screened / QC no positives
| Gene | Gpr45 |
| Chromosome | 1 |
| Deletion Start | 43,032,182 |
| Deletion End | 43,033,324 |
| Deletion Size | 1,143 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 43,032,286 | 43,032,305 | TCTGCACCGCTCAGGATCTC |
| TUP | 43,032,347 | 43,032,370 | TGATGCAGACCACCGTGTTGCCAA |
| TUR | 43,032,373 | 43,032,391 | TGGCTGGCCTCTGGTACAC |
| TDF | 43,032,579 | 43,032,598 | CATTAGCGTGGACCGGTTTC |
| TDP | 43,032,600 | 43,032,623 | CATCATCGTGCAGCGTCAGGACAA |
| TDR | 43,032,625 | 43,032,643 | TAGCCCTGCGTGGGTTCAG |
PCR Primers
| Primer | Sequence |
| SU | AAGCCCATGGCTACCACAG |
| SD | CTGGCCGACTTCTGCAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 337 bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
