10154 Rnf152 Status: Germline transmission achieved
| Gene | Rnf152 |
| Chromosome | 1 |
| Deletion Start | 107,181,404 |
| Deletion End | 107,180,776 |
| Deletion Size | 629 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 107,181,169 | 107,181,189 | AGAGGTCTTGGCAGTCATTGC |
| TUP | 107,181,139 | 107,181,165 | ACCACACACTTCCGAGCATACCCCAGT |
| TUR | 107,181,112 | 107,181,132 | CACCCATTGCTTGGAAGTTTG |
| TDF | 107,180,911 | 107,180,931 | CGTGGTGAAGAGCTCCACATG |
| TDP | 107,180,884 | 107,180,907 | ACCAGGATGACAGTGCACACGCCA |
| TDR | 107,180,856 | 107,180,876 | GCCCAGGAGGAAGACCAAAAC |
PCR Primers
| Primer | Sequence |
| SU | GGAAACCTATGGTCTTACCAG |
| SD | CCTGGGATCTTGCTGAGAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 389 bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 261 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10154B-C4 | VGB6 | C57BL/6NTac | |
| 10154B-E8 | VGB6 | C57BL/6NTac | |
| 10154B-E12 | VGB6 | C57BL/6NTac | |
| 10154B-F8 | VGB6 | C57BL/6NTac | |
| 10154B-G3 | VGB6 | C57BL/6NTac | |
| 10154B-H6 | VGB6 | C57BL/6NTac | |
