10157 Slamf8 Status: Cancelled
| Gene | Slamf8 |
| Chromosome | 1 |
| Deletion Start | 172,590,429 |
| Deletion End | 172,582,425 |
| Deletion Size | 8,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 172,590,328 | 172,590,348 | CCAGCTGCCTTCATCCTACTG |
| TUP | 172,590,288 | 172,590,318 | TTGCCTCTCCAAACCAAGTAAGAATCAAGGA |
| TUR | 172,590,259 | 172,590,279 | GCTTGCTCCACACCTCACTTC |
| TDF | 172,582,732 | 172,582,752 | TCTGCCACACGACTGTTTGTC |
| TDP | 172,582,699 | 172,582,724 | AAGGCACATCGTGGCGCTAACTCATG |
| TDR | 172,582,669 | 172,582,689 | TTAGTGTGTGCACGGCATCTG |
PCR Primers
| Primer | Sequence |
| SU | TGACTTACAAGAACCGGCTC |
| SD | CTAGTTCACCTTGCCCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 90 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
