10158 Il1f6 Status: Cancelled
| Gene | Il1f6 |
| Chromosome | 2 |
| Deletion Start | 24,215,829 |
| Deletion End | 24,224,597 |
| Deletion Size | 8,769 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,216,091 | 24,216,111 | GCCCCTCTGGCTAATGTTGTG |
| TUP | 24,216,116 | 24,216,143 | TGTCCTCGGCCTTTCATTTTACCACACA |
| TUR | 24,216,145 | 24,216,167 | CTCCAGATACGGGCAGTTAACAC |
| TDF | 24,224,500 | 24,224,521 | TCATCGCTGTCTGCTCTAAAGG |
| TDP | 24,224,523 | 24,224,548 | AGCTGCCCACTCATTCTGACCCAAGA |
| TDR | 24,224,555 | 24,224,578 | TCGAAGTCAGTGATGAAGATTTCC |
PCR Primers
| Primer | Sequence |
| SU | TGACCTTGGTGCTCTCTGTC |
| SD | GCAAAGGTGAGGTTCTCTGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 367 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 133 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
