10162 Commd3 Status: Cancelled
| Gene | Commd3 |
| Chromosome | 2 |
| Deletion Start | 18,672,449 |
| Deletion End | 18,675,926 |
| Deletion Size | 3,478 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,672,806 | 18,672,826 | CCCTGCCAGACTGTTTCTTTG |
| TUP | 18,672,837 | 18,672,865 | AGGCCACAAACTAGACTTGAACACGGCTT |
| TUR | 18,672,913 | 18,672,933 | TCCCCAGTTGAAGTCCAAGTG |
| TDF | 18,675,697 | 18,675,718 | GGACCAGATCCAGCAGAAGAAG |
| TDP | 18,675,726 | 18,675,750 | TTTCCAAAGGGCTTTGCGAGCTGTT |
| TDR | 18,675,767 | 18,675,790 | CAGTCGGAAGGAAGAACACCTAAG |
PCR Primers
| Primer | Sequence |
| SU | CACTCAGTCTCCCGTTGTCC |
| SD | GCTCAAGATAGAAGGAACGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 538 bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | 319 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
