10168 Dpp7 Status: Germline transmission achieved
| Gene | Dpp7 |
| Chromosome | 2 |
| Deletion Start | 25,211,844 |
| Deletion End | 25,207,918 |
| Deletion Size | 3,927 |
| Genome Build | 37 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,211,741 | 25,211,761 | CCAGGCCAGAGGTGAGTACAG |
| TUP | 25,211,708 | 25,211,732 | CCCTTCCTCCTGACCCGCCTAGATC |
| TUR | 25,211,686 | 25,211,706 | GGAGCTGGTTGGGATCATCAG |
| TDF | 25,208,033 | 25,208,053 | GGAGGTGCGTAAGCTGGAATC |
| TDP | 25,208,001 | 25,208,026 | TGGCTGCTGCTACCCACTCACGAATG |
| TDR | 25,207,978 | 25,207,998 | TGGCATTGCTGGTTGCTTTAG |
PCR Primers
| Primer | Sequence |
| SU | AGCTGGACACTTGGGAGATC |
| SD | CCCAGTAGAGGCAGGTTTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 386 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 377 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10168A-F4 | VGB6 | C57BL/6NTac | |
| 10168A-A8 | VGB6 | C57BL/6NTac | |
