10170 Slc35c1 Status: Cancelled
| Gene | Slc35c1 |
| Chromosome | 2 |
| Deletion Start | 92,459,156 |
| Deletion End | 92,454,170 |
| Deletion Size | 5,005 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,458,853 | 92,458,871 | CTCAAGGTGGCCCGAAGTG |
| TUP | 92,458,825 | 92,458,850 | CCGATAAAGACCACTGACAGCGGCAG |
| TUR | 92,458,759 | 92,458,779 | GAGCGTCCCACGTTGTAGAAG |
| TDF | 92,454,617 | 92,454,637 | CACAGTATCTGGCGCCTAACC |
| TDP | 92,454,581 | 92,454,605 | TGTCAATGCCTGCGTGCTCTTCTTG |
| TDR | 92,454,558 | 92,454,577 | TCGCCCAGCACTATCATCAG |
PCR Primers
| Primer | Sequence |
| SU | GGCAAATCCGGAAGCAGAAC |
| SD | GACCATATGGAGGTAGACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 310 bp | - | + | + |
| TUF | TUR | 113 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 317 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
