| Gene | Chst1 |
| Chromosome | 2 |
| Deletion Start | 92,613,171 |
| Deletion End | 92,614,455 |
| Deletion Size | 1,285 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,613,245 | 92,613,264 | ACAGCCATCCGCACTTTCAC |
| TUP | 92,613,270 | 92,613,295 | AGTCTTTCCACACTTGCCCTGGGCTG |
| TUR | 92,613,297 | 92,613,317 | GCTCTGCCAAGCCAGTATCTG |
| TDF | 92,614,265 | 92,614,283 | CGCTTCCGCCTCTCCTATG |
| TDP | 92,614,293 | 92,614,314 | CCTTTGCCCAGAATGCCTGCCA |
| TDR | 92,614,319 | 92,614,338 | TAGCCCAGCTGAGCCAACAC |
PCR Primers
| Primer | Sequence |
| SU | CCTCTGCAACCACAACTCTC |
| SD | AAGGACACACAGATGGTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 366 bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 228 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10172A-D6 | VGB6 | C57BL/6NTac | |
| 10172B-A7 | VGB6 | C57BL/6NTac | |
| 10172B-D12 | VGB6 | C57BL/6NTac | |
