| Gene | Tapt1 |
| Chromosome | 5 |
| Deletion Start | 44,226,600 |
| Deletion End | 44,204,218 |
| Deletion Size | 22,383 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 44,226,057 | 44,226,077 | TCAATGCCGAGGTGACTCTTC |
| TUP | 44,226,025 | 44,226,055 | CCGAGACTCAACTTCGCATAAAACCATAGGG |
| TUR | 44,225,999 | 44,226,018 | GATTCGGCGGGCAAGTTAAC |
| TDF | 44,205,572 | 44,205,594 | TCGTGGAGGGAAGACTTAAACTG |
| TDP | 44,205,541 | 44,205,567 | CTTGCACGTGACTTAGGCTCACACCGT |
| TDR | 44,205,511 | 44,205,535 | CATTCTCGACTCATGAACAACAATG |
PCR Primers
| Primer | Sequence |
| SU | CGACCCGGCGGCATGGAG |
| SD | GAACAGATAGGCAGTGTATGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 84 bp | + | + | - |
| NeoFwd | SD | 247 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10176C-C4 | VGB6 | C57BL/6NTac | |
| 10176A-G12 | VGB6 | C57BL/6NTac | |
