10178 Rprm Status: ES cell colonies screened / QC no positives
| Gene | Rprm |
| Chromosome | 2 |
| Deletion Start | 54,085,301 |
| Deletion End | 54,084,955 |
| Deletion Size | 347 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 54,085,249 | 54,085,268 | GGCCTGTTCCTGGTCAACAG |
| TUP | 54,085,177 | 54,085,196 | ACCGACGATGGCTTCGCCGA |
| TUR | 54,085,132 | 54,085,152 | TGCACCACACGCATGATGTAC |
| TDF | 54,085,128 | 54,085,147 | TCATGCGTGTGGTGCAGATC |
| TDP | 54,085,097 | 54,085,123 | CCACAGTGAGCGAGAGCACACACATGA |
| TDR | 54,085,069 | 54,085,089 | TTGCAGCCAAGGAAGAAGATG |
PCR Primers
| Primer | Sequence |
| SU | CGCCAAGTTCCAAGAGCTCC |
| SD | TGCGCCCTGCTTTTGCCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 385 bp | - | + | + |
| TUF | TUR | 137 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 332 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
