10179 Ermn Status: Cancelled
| Gene | Ermn |
| Chromosome | 2 |
| Deletion Start | 58,052,713 |
| Deletion End | 58,047,734 |
| Deletion Size | 4,980 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,052,674 | 58,052,693 | GAGCGGGACAGAGTGCAATG |
| TUP | 58,052,644 | 58,052,670 | ACAGACCACCTGAGAATGGCCAACAGC |
| TUR | 58,052,617 | 58,052,637 | TTGTCTCCTGCCTGGTTTGAC |
| TDF | 58,048,219 | 58,048,241 | CGAACAGCAGTAACCTGGAAGTG |
| TDP | 58,048,174 | 58,048,198 | TGCCCAGCAACAGCTTGAGCAGAGA |
| TDR | 58,048,149 | 58,048,172 | CGATTTCAGTTGTACTCGCATCTC |
PCR Primers
| Primer | Sequence |
| SU | GCTGCGAGGTGGTGGGGATG |
| SD | TAAGCAAAAACCCAGGAATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 346 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 290 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
