10180   Disc1     Status: Cancelled

GeneDisc1
Chromosome8
Deletion Start125,054,214
Deletion End125,088,439
Deletion Size34,226
Genome Build38
MGI IDMGI:2447658
NCBI Gene ID244667
BAC ID94j21
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableNo

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF125,054,607125,054,629GGTTGAGAGAGGTGTGGGAACTG
TUP125,054,664125,054,688CGCGTCACAGTCCACACTCGGAAGT
TUR125,054,718125,054,739CCCAAGCTGACAGAGGAAATGG
TDF125,088,129125,088,149CCCAAGAGATGGCAGCTGAAG
TDP125,088,151125,088,175ATCTAGCTCAGACAGGCCCCACGGG
TDR125,088,177125,088,196GGTCCAGAGATGCCGAGGAT

PCR Primers

PrimerSequence
SUTCCTTGGTTTCCTCCAAGTG
SDTGCAGGTCAAAGGTTGTGGAG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev352 bp-++
TUFTUR133 bp++-
TDFTDR68 bp++-
NeoFwdSD339 bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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