10183 Bmyc Status: Cancelled
| Gene | Bmyc |
| Chromosome | 2 |
| Deletion Start | 25,706,912 |
| Deletion End | 25,707,455 |
| Deletion Size | 544 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,706,956 | 25,706,976 | AACCGCGACCTGGACTATGAC |
| TUP | 25,706,978 | 25,707,002 | CGGTGCAGCCATACTTCATGTGCGA |
| TUR | 25,707,004 | 25,707,023 | TGCACGTCCTCCTCCTCATC |
| TDF | 25,707,216 | 25,707,234 | AGGAGGAGACGCGGTGAAG |
| TDP | 25,707,246 | 25,707,269 | CTGCGACCCGGATGACGAGACCTT |
| TDR | 25,707,302 | 25,707,320 | GAGGCCGAGAAGCCATTCC |
PCR Primers
| Primer | Sequence |
| SU | GTCTGGCGACGAGCTGAC |
| SD | AATCTTGTATAACTGATAAGACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 305 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | 304 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
