10187 Entpd2 Status: Cancelled
| Gene | Entpd2 |
| Chromosome | 2 |
| Deletion Start | 25,395,956 |
| Deletion End | 25,400,973 |
| Deletion Size | 5,018 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,396,292 | 25,396,311 | GGCAAAGTTCGTGGGAATTC |
| TUP | 25,396,314 | 25,396,337 | AGCTCCATCGCGAAGCCGAAAGAA |
| TUR | 25,396,339 | 25,396,357 | AGCTCCTCTGCCGCTCTTG |
| TDF | 25,400,851 | 25,400,870 | CGTAAGGGCACCCACTTCAG |
| TDP | 25,400,872 | 25,400,895 | TCCTGGGTTGCTCTCCTGCTGCTC |
| TDR | 25,400,905 | 25,400,923 | CCAAAGCCGCCAAGATCAG |
PCR Primers
| Primer | Sequence |
| SU | AGAACGTGACTGATAGCTGTG |
| SD | CCCAGACAGAAAGTACCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 335 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
