10189 Fcna Status: Cancelled
| Gene | Fcna |
| Chromosome | 2 |
| Deletion Start | 25,627,538 |
| Deletion End | 25,624,821 |
| Deletion Size | 2,718 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,627,187 | 25,627,205 | CTGAGCCCACACACCATCC |
| TUP | 25,627,160 | 25,627,184 | CCCTATGTCCAGATGCATGCCTGCC |
| TUR | 25,627,139 | 25,627,158 | ATGCCCAGCCTGTAGGAAGG |
| TDF | 25,624,941 | 25,624,959 | TCAACGGGCGCTACTTGTC |
| TDP | 25,624,912 | 25,624,937 | TGCCATCCGCATAACTCTCATGGGAG |
| TDR | 25,624,883 | 25,624,902 | GTGGTGACCTTGGCCAGTTC |
PCR Primers
| Primer | Sequence |
| SU | GACTGCTCTGTCTCTGTCCC |
| SD | AGGCCACCATGGATGTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 476 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
