10191 Qrfp Status: Cancelled
| Gene | Qrfp |
| Chromosome | 2 |
| Deletion Start | 31,808,915 |
| Deletion End | 31,808,525 |
| Deletion Size | 391 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 31,808,847 | 31,808,866 | TTCCCCTGCTGGACAGAAGG |
| TUP | 31,808,812 | 31,808,835 | ATCCTGGCTCCGATGTCACCGATG |
| TUR | 31,808,760 | 31,808,780 | TGGATTTTGAACCGAGTTGGG |
| TDF | 31,808,590 | 31,808,609 | CTGGCAGAGGAGCTGAGCAG |
| TDP | 31,808,564 | 31,808,588 | TGAAGCCTCCCTTCCTCCGGCTGTA |
| TDR | 31,808,543 | 31,808,562 | CTCACCGTCCAAAGCGGAAG |
PCR Primers
| Primer | Sequence |
| SU | TCTGGTATTCCAGTACTGAG |
| SD | GAACACCACCCAGATTTCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 371 bp | - | + | + |
| TUF | TUR | 107 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | 246 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
