10192 Ccbl1 Status: Cancelled
| Gene | Ccbl1 |
| Chromosome | 2 |
| Deletion Start | 30,194,103 |
| Deletion End | 30,185,504 |
| Deletion Size | 8,600 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,193,821 | 30,193,841 | ACCTTGTGGACTGGCAATGAC |
| TUP | 30,193,798 | 30,193,819 | CCCAATCAGGCAGCAGGCCATG |
| TUR | 30,193,759 | 30,193,780 | AGCCGTAGCTATGGAGGATCAG |
| TDF | 30,185,967 | 30,185,987 | GCTGTGAACCCTGCTCAAACC |
| TDP | 30,185,932 | 30,185,958 | TCAAACAGTTCTTCCCAGCTCTGCCCA |
| TDR | 30,185,895 | 30,185,914 | CCTCCCAGGGTGCAGACAAC |
PCR Primers
| Primer | Sequence |
| SU | GGAGAACAGGTCCCAGCTAG |
| SD | TTACTGTCCCAGGCAGAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 298 bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 93 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
