10193 Mrps2 Status: Cancelled
| Gene | Mrps2 |
| Chromosome | 2 |
| Deletion Start | 28,468,103 |
| Deletion End | 28,470,008 |
| Deletion Size | 1,925 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 28,468,489 | 28,468,509 | GCTCTCGAAACCCGTGAAGAC |
| TUP | 28,468,515 | 28,468,545 | TTTGTATCTGTCCATCTTGCTGGTCTTCTGG |
| TUR | 28,468,551 | 28,468,570 | GACTTCGGGTGCCTTAAACG |
| TDF | 28,469,772 | 28,469,790 | ATCCCCACAGTGGGCATTG |
| TDP | 28,469,797 | 28,469,823 | CCAACTGCAACCCATGCCTCATCACTT |
| TDR | 28,469,826 | 28,469,844 | CGTCGTTGCCAGGGATAGG |
PCR Primers
| Primer | Sequence |
| SU | TCGCCTCCTCCAGAGGATGCTG |
| SD | CTATGTTCAAGTCTTAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 409 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | 326 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
