10198 1700019L03Rik Status: Cancelled
| Gene | 1700019L03Rik |
| Chromosome | 2 |
| Deletion Start | 32,784,384 |
| Deletion End | 32,777,479 |
| Deletion Size | 6,906 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 32,784,195 | 32,784,214 | GGCCAGCAGGGAAGCTAGTG |
| TUP | 32,784,165 | 32,784,193 | TACCAGGCCAAATGCACACAGGTTCAGAT |
| TUR | 32,784,140 | 32,784,159 | GCCAGAGCCCAGGTCTAAGG |
| TDF | 32,778,248 | 32,778,267 | AGGACGGCGACTTTGATGTG |
| TDP | 32,778,221 | 32,778,244 | TTCCAACTGCAGCGCAAGGAGCTG |
| TDR | 32,778,187 | 32,778,206 | CCCCTGAGCTGAGCAATACC |
PCR Primers
| Primer | Sequence |
| SU | TGGAACCCAAACTTGTACTC |
| SD | AAGCCAAGACAGGGCCACAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 311 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 81 bp | + | + | - |
| NeoFwd | SD | 230 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
